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KMID : 0614619930250010107
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Korean Journal of Gastroenterology
1993 Volume.25 No. 1 p.107 ~ p.115
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Prevalence and Clinical Significance of Precore HBV Mutants in Patients with Chronic Liver Disease B
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Abstract
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In order to assess the prevalence of precore HBV mutants which frequently have been found in a sigificant proportion of HBeAg negative patiets with chronic liver disease B and its clinical significance, we ampllfied the partial segment of HBV DNA
containing the entire precore region using PCR and Hybridized the amplified products with three oligonuclotide (18 mer) probes, complementary to non-mutated, one (1896, G to A) or two (1896 and 1899 G to A) point-inutated distal precore sequences
respectively in 39 patients (25 HBeAg negative and 14 HBeAg positive cases).
HBV DNA was detected in 12 (48%) of 25 patients negative for HBeAg and 6 (50%) of these 12 showed precore HBV mutants. In 14 patients positive for HBeAg. HBV DNA was detected in 11 (78.6%) and only one (9%) of them was precore HBV mutant. In 25
HBeAg
negative patients. 14 patients showed abnormal LFT. HBV DNA was detected in 9 (64.3%) and 6 (66.7%) of these 9 were precore HBV mutants. However, HBV DNA was detected only 3 (27.3%) of 11 patiente with normal LFT and all were wild type HBV. All
of
6
patiets with precore HBV mutants had 1896 G to A one point mutation. but only 2 of them had twe point inutation as the mixed form.
In conclusion, precore HBV mutants were detected in a significant proportion of HBeAg negative patients with chronic liver disease B, especially in those who had abnormal LFT. These results may suggest that viral heterogeneity affect the severity
of
chronic liver disease B.
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